Journal: American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons

Article Title: Comprehensive Assessment and Standardization of Solid Phase Multiplex-Bead Arrays for the Detection of Antibodies to HLA

doi: 10.1111/ajt.12287

Figure Lengend Snippet: Kits and reagents

Article Snippet: Lot No. One Lambda, Inc. LSM12 LABScreen Mixed Class I and Class II Scr 017 016 LS1PRA LABScreen PRA Class I PRA1 014 013 LS2PRA LABScreen PRA Class II PRA2 013 012 LS1A04 LABScreen Single Antigen HLA Class I SA1 006 007 LS2A01 LABScreen Single Antigen HLA Class II SA2 008 009 LS-NC LABScreen negative control 010 011 LS-AB2 Goat anti-human IgG A76 A76 Gen-Probe, Inc 628215 Lifecodes LifeScreen Deluxe Scr 100510-LMX 052311-LMX 628200 Lifecodes Class I ID PRA1 102710-LM1 041311-LM1 628223 Lifecodes Class II IDv2 PRA2 090210-LM2Q 042211-LM2Q 265100 Lifecodes LSA Class I SA1 10050M 04101P 265200 Lifecode LSA Class II SA2 10190D 05311A Luminex L100-CAL1 Calibrator Microspheres I B21094 L100-CAL2 Calibrator Microspheres II B20676 L100-CON1 Control Microspheres I B20770 L100-CON2 Control Microspheres II B20501 Open in a separate window Kits and reagents

Techniques: Negative Control, Luminex

Journal: Transplant international : official journal of the European Society for Organ Transplantation

Article Title: Antibody-mediated rejection despite inhibition of terminal complement.

doi: 10.1111/tri.12396

Figure Lengend Snippet: Figure 4 Comparison of levels of antidonor antibody during the first month post-transplantation demonstrates significantly higher levels in rejecters for DSA IgM (a), but no difference in DSA IgG (b); DSA C1q (c); or DSA IgG3 (d). Patients with rejection are with full diamonds, the one patient with no rejection, but accelerated transplant glomerulopathy is in open diamond, and the nonrejecters are in closed circles.

Article Snippet: IgG3 HLA antibodies were tested in all patients by Terasaki Foundation using LABscreen SAB kits and a secondary anti-human IgG3 PE-conjugated antibody (IgG3 clone HP6050, Southern Biotech, Birmingham, AL, USA) [4].

Techniques: Comparison, Transplantation Assay

Journal: Transplant international : official journal of the European Society for Organ Transplantation

Article Title: Antibody-mediated rejection despite inhibition of terminal complement.

doi: 10.1111/tri.12396

Figure Lengend Snippet: Figure 5 Anti-HLA antibody patterns in patients who developed early AMR and one without AMR. (a) AMR Patient #1 DSA Levels. Increased levels of DSA IgM (anti-B8 and anti-Cw7) at day 7 correlating with early AMR (solid arrow). Plasma exchange started on Day 7 rapidly reversed the AMR episode and led to reduced IgM DSA but IgG DSA continued to rise. (b) AMR Patient #2 DSA Levels. Increase in DSA IgM at day 7 cor- relating with early peritubular capillaritis (dashed arrow) and then sub- sequent early AMR (solid arrow) in patient 2. Again, the AMR episode was easily treated with plasma exchange and IgM quickly was reduced while IgG DSA continued to rise. (c) High levels of IgG DSA without IgM DSA in a patient who did not develop AMR. Typical DSA pattern in a patient with persistently high IgG DSA and C4d deposition on surveil- lance biopsy who did not develop AMR and did not have IgM DSA.

Article Snippet: IgG3 HLA antibodies were tested in all patients by Terasaki Foundation using LABscreen SAB kits and a secondary anti-human IgG3 PE-conjugated antibody (IgG3 clone HP6050, Southern Biotech, Birmingham, AL, USA) [4].

Techniques: Clinical Proteomics